PCR cycle selection is a delicate balancing act. When preparing RNA-seq libraries, choosing too many cycles risks overcycling, which can compromise data quality and distort transcript representation.

RNA sequencing (RNA-seq) is widely used in biomedical research, advancing our understanding of gene expression across biological systems. Traditional methods require upstream RNA extraction from ...

Next-generation sequencing is a powerful technology that has ushered in a Cambrian era of genomic research by enabling high-throughput, cost-effective DNA and RNA sequencing. DNA sequencing of entire ...

Understand the stages of a standard RNA-seq bioinformatics workflow, including the role of QC, alignment, gene expression quantification, and specialized application analyses Identify key QC metrics ...

New technologies advance scalable library normalization for high-throughput genomics labs and improved amplification accuracy for oncology-focused sequencing workflows where data quality is critical ...

Advances in next-generation sequencing (NGS) have propelled genomics research to new heights. With the introduction of new sequencing platforms such as Element Biosciences’ AVITI and Ultima Genomics’ ...

A comprehensive review article titled “Bioinformatics perspectives on transcriptomics: A comprehensive review of bulk and single-cell RNA sequencing analyses,” published in Quantitative Biology, ...